アカデミックプラザ2013

研究概要

Optimization of antioxidant activity of Pinus koraiensis extracts

研究機関名

Daegu University, Korea
Duksung Women’s University, Korea

代表者

Dr. Jun Ho Lee (Professor in Food Engineering, Daegu University)

本研究の主旨

Pinus koraiensis, a large conifer that has important economical importance, is found mainly across Korea, Japan, and the north-eastern part of China, and the seeds of P. koraiensis have been used as a food supplement for a long time (Lee et al., 2008). The plant has also been used in oriental fork medicine having effect on releasing fatigue, anti-ageing, and anti-inflammatory (Watanabe et al., 1995). It has been also reported that Pinus bark extract exhibits anti tumor, antioxidant, anti aging, and anti mutation activities based on removing superfluous free radicals and enhancing immunity (Asset et al., 1999; Bagchi et al., 2000; Li et al., 2007). Not to mention, Pinus plants have a high oil content (Sugano et al., 1994; Wolff & Bayard, 1995) and contain all essential amino acids and essential fatty acids (Han & Hwang, 1990).
In this study, important extraction conditions such as ethanol concentration, sample ratio, and extraction temperature were optimized using response surface methodology (RSM), by employing a five-level, three-variable central composite rotatable design (CCRD), in order to obtain the optimal condition for the extraction of electron donating ability and coumaric acid content from Pinus koraiensis.
To examine the effects of extraction conditions for electron donating ability and coumaric acid content from Pinus koraiensis, response surface plots were constructed. Electron donating ability ranged from 50.56-79.51% and coumaric acid content from 10.98-33.72 mg%, respectively. For optimum conditions, refer to the table below.

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Table 1. Process variables and their levels used in the central composite rotatable design for analysis of P. koraiensis extracts

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Table 2. Central composite design matrix for the experimental design and experimental results

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Table 3. The optimum extraction condition of P. koraiensis by superimposed response surfaces

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Fig. 1. Response surfaces for electron donating ability (A) and coumaric acid content (B) of P. koraiensis as a function of extraction temperature, ethanol concentration and solvent to sample ratio.

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Fig. 2. Superimposed response surfaces for optimized extraction conditions of electron donating ability and coumaric acid content from P. koraiensis as a function of extraction temperature, ethanol concentration and solvent to sample ratio.

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